High quality human iPSC lines for research and commercial use


In collaboration with top stem cell researchers in China and US, CIB has made a large collection of high quality iPS cell lines that are suitable for basic research and for drug discovery. All of these iPS cell lines have been produced under a stringent quality control system following standard operation protocols and have met the international standards.


iPSC lines derived from normal non-diseased donors:

These iPSC lines can be used for multiple applications: 1) controls for development of new reprogramming methods; 2) starting materials for optimization of differentiation protocols; 3) source materials for study of developmental biology, epigenetics, proteomics, etc. and 4) parental cell lines for further genetic modifications.


Cat#

Source

Order

iPSN-0004

Urine epithelial cell

request

iPSN-0039

Umbilical cord mesenchymal tissue-1

request

iPSN-0005

Umbilical cord mesenchymal tissue-2

request

iPSN-0006

Amniotic mesenchymal tissue

request

iPSN-0007

Chorionic mesenchymal tissue

request

iPSN-0008

Amniocytes

request

iPSN-0009

Skin fibroblasts-1

request

iPSN-0010

Skin fibroblasts-2

request

iPSN-0011

Humerus periosteum tissue

request

iPSN-0012

Femur periosteum tissue

request

iPSN-0014

Ulna periosteum tissue

request

iPSN-0015

Tibia periosteum tissue

request


iPSC lines derived from donors with genetic diseases:

These iPSC lines can be used as tools for study of disease mechanism at cellular and molecular levels, for discovery of novel therapeutics to treat the underlying diseases, and for exploration of cell replacement therapy or gene therapy.


Cat#

Source

Order

iPSD-0016

Skin fibroblasts of donor with Wilson's disease-1

request

iPSD-0017

Skin fibroblasts of donor with Wilson's disease-2

request

iPSD-0018

Skin fibroblasts of donor with Wilson's disease-3

request

iPSD-0019

Skin fibroblasts of donor with Wilson's disease-4

request

iPSD-0020

Skin fibroblasts of donor with neurofibromatosis-1

request

iPSD-0021

Skin fibroblasts of donor with neurofibromatosis-2

request

iPSD-0022

Skin fibroblasts of donor with neurofibromatosis-3

request

iPSD-0023

Amniocytes of donor with beta thalassemia-1

request

iPSD-0024

Amniocytes of donor with beta thalassemia-2

request

iPSD-0025

Urine epithelial cells of donor with Parkinson’s disease

request

iPSD-0026

Skin fibroblasts of donor with Parkinson’s disease-1

request

iPSD-0027

Skin fibroblasts of donor with Parkinson’s disease-2

request

iPSD-0028

Skin fibroblasts of donor with Alzheimer’s disease

request

iPSD-0032

Skin fibroblasts of donor with Ehlers-Danios syndrome

request

Isogenic iPSC lines

These iPSC lines are derived from a single parental iPSC line, genetically engineered to mimic known disease-causing mutations. Compared to iPS cell lines derived from diseased patients, these isogenic iPSC lines are invaluable materials for studying gene function in a near-identical genetic background, and thus enable researchers to conduct in-depth study of disease mechanisms. These materials are most useful for studying genes that have small effect on phenotype because an isogenic set containing wild type, mono-allelic KO/KI, and bi-allelic KO/KI can be studied simultaneously.



Cat#

Product description

Order

iPSM-0033

 Park2 bi-allelic knockout : Park2 -/-

request

iPSM-0033m

  Park2 mono-allelic knockout: Park2 +/-

request

iPSM-0034

BDNF bi-allelic knockout: BDNF -/-

request

iPSM-0034m

BDNF mono-allelic knockout: BDNF +/-

request

iPSM-0035

Park7 bi-allelic knockout: Park7 -/-

request

iPSM-0035m

 Park7 mono-allelic knockout: Park7 +/-

request

iPSM-0036

Lrrk2 bi-allelic knockout: Lrrk2 -/-

request

iPSM-0036m

 Lrrk2 mono-allelic knockout: Lrrk2 +/-

request

iPSM-0037

Pink1 bi-allelic knockout: Pink1-/-

request

iPSM-0037m

Pink1 mono-allelic knockout: Pink1 +/-

request

iPSM-0038

NBN bi-allelic knockout: NBN -/-

request

iPSM-0038m

Mono-allelic knockout: NBN +/-

request


Standard QC tests of CIB iPSC lines:


1) qPCR tests for transgene silencing and for expression of endogenous transcription factors.

2) Immunocytochemistry tests for pluripotency biomarkers

3) Karyotype analysis

4) Embryoid body (EB) formation 

5) qPCR analysis of germ layer-specific biomarkers

6) Cell identification (STR analysis)

7) Sterility and mycoplasma tests

8) Additional information includes sequence analysis if the iPSC contains known mutations associated with certain diseases. 

 

Examples of CIB iPSC characterization:

1) iPSC derived from normal non-diseased donors

                                             

 

Figure 1.  A. Expression of transgens; B. Expression of endogenous Oct4 and Nanog; C. Surface markers for pluripotency; D. Karyotye; E. Embryoid body formation (morphology and expression of germ layer-specific biomarker genes); F. Demethylation of Oct4 and Nanog promoters; G. Teratoma formation in mouse.

 

2) iPSC derived from donors with genetic diseases (additional genotyping):

 

 


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